Comparison of protection and release behavior of different capsule polymer combinations based on L. acidophilus survivability and function and caffeine release

• The performance of 10 single or in DUOCAP® combination delivery systems was evaluated vitro SHIME model. Viability L. acidphilus and caffeine release were used as markers under fed fasted conditions. DR-in-DR DR-in-VC showed the slowest release. prevented acidophilus damage upper GIT. Delivery DUOCAP changed short chain fatty acid profile colonic Oral administration active pharmaceutical ingredients, nutraceuticals, enzymes probiotics requires an appropriate system for optimal bioactivity absorption. harsh conditions during gastrointestinal transit can degrade administered products, hampering their efficacy. Enteric delayed-release formulations may help overcome these issues. In a Simulator Human Intestinal Microbial Ecosystem model (SHIME) using marker kinetics survivability indicator protection, we compared ten capsule configurations, combinations. function its impact on gut microbiota further tested three selected types, combinations DRcaps® (DR-in-DR) Vcaps® (DR-in-VC) Plus We found that stomach small intestine conditions, led to best both based slow highest survivability. however, quickest probiotic When DR-in-DR, capsules through whole tract, including be slower containing capsules. addition, survival significantly increased formulation capsule. To assess microbial function, acetate, butyrate propionate well ammonia measured. released from induced significant increase decrease ammonia, suggesting proliferation butyrate-producing bacteria reduction ammonia-producing bacteria. These data suggest included reaching colon is viable functional, potentially contributing changes composition diversity.